E.coli相关论文
RegⅢγ（Regenerating gene,Reg）是一种新型肠道抗菌蛋白,具有抗菌、抗炎、损伤修复和促进机体不同组织细胞增殖的作用,对维持肠道菌......
本论文为了提高胆固醇氧化酶工程菌的表达水平，并建立简便、高效的分离纯化工艺，总体上提高胆固醇氧化酶的提取率。考虑到His-tag的......
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Biological Effect Studies of Norfloxaein Complexes on Tetrahymena and Escherichia Coli by Microcalor
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Adhesion of bacteria to surfaces is an essential process in life,and involved in the infection and the organization of b......
Conventional TEM has been widely used in characterization and analysis of inorganic, organic and biological samples.It g......
Antibacterial activity of photocatalytic materials is primarily induced by UV light irradiation.The Bi2O2CO3/Bi3NbO7 (Bi......
Investigation of Inhibitory Factors on Ethanol Fermentation of Xylose by Recombinant Escherichia col
Hemicellulose is a potential by-product that is presently under-utilized in the papermaking industry.It is a hetero-carb......
[目的]本研究采用非标记定量蛋白质组学技术对构建的黏菌素耐药E.coli进行比较研究，从蛋白质水平探讨细菌耐药产生的蛋白谱变化，筛选......
Bacterial attachment to solid surfaces and the subsequent formation of biofilms increase both health care costs and econ......
Efficient Production of Fatty Alcohols by E.coli Modified with Tandem Repeats Assisted Genome Editin
Genome editing can introduce predetermined sequence changes to the target genome.In this work,a new genome editing metho......
The hydrodynamic cavitation water treatment process can effectively remove organic matter and microorganisms based on th......
Fermentation of Xylose to Succinate by Enhancement of ATP Supply in Metabolically Engineered Escheri
In Escherichia coli K12, succinate was not the dominant fermentation product from xylose.To reduce byproduct formation a......
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Proteome-wide Quantification of Escherichia Coli Lysine Acetylation in response to glucose utilizati
Lysine acetylation (Kac) and its regulatory enzymes are known to have pivotal roles in mammalian cellular physiology,suc......
The increasing use of zinc oxide nanoparticles(ZnO NPs)is likely to increase their release to the aquatic environment,an......
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Potential influences of the segments in 16S rRNA besides anti-SD sequence on gene translation initia
Background: Anti-SD (Shine Dalgarno) sequence of 16S rRNA is considered a key segment to regulate gene translation initi......
Objectives: Toll/interleukin 1 receptor domain containing protein encoded by E.coli (TcpC) was found to be a virulence f......
Objectives: Maltose binding protein (MBP),a 42 kDa protein part of the maltose/maltodextrin system of E.coli,is often fu......
Background: Extended-spectrum β-1actamases (ESBLs) is prevalent due to the extensive usage of the extended-spectrum cep......
Bioavailability is the key component to evaluate the environmental fate of organic contaminants and their impacts to hum......
We proposed new counting method of the number of active bacterium by using micro-droplet as an incubator (figure 1).Duri......
美国FDA于2021年6月30日批准Jazz制药公司(Jazz Pharmaceuticals)的Rylaze[asparaginase erwinia chrysanthemi(recombinant)-rywn......
Wild-type MIC Distribution and Epidemiological Cut-off Value and Resistant Characteristics of Colist
The aim of the present study was to investigate minimum inhibitory concentration(MIC)distributions by broth microdilutio......
Photocatalysis is a promising water purification method due to its potential of using solar energy directly and achievin......
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Astaxanthin as a highly valued keto-carotenoid has kinds of beneficial effects on human health,including cancer prev......
In Search for a Fast and Reliable Detection Method of Chemical Properties Activating Prophage Replic
In the genome of bacteria dormant bacteriophages(called prophages)can be activated by chemicals.These bacteriophages......
蓝靛忍冬作为新兴的小浆果,其果实的化学成分对病原菌抑菌性鲜有报道.本文对蓝靛忍冬果实组分进行提取分离并检测各分离组分对......
分别以水煮法灭活E.coli O157：H7制备菌体抗原、热酚水法提取脂多糖(LPS)和乙酸水解LPS制备O抗原多糖(OPS)3种抗原免疫产蛋母鸡4次......
通过ＤＮＡ体外重组技术，以ｐＥＴ－３ｂ为表达载体，构建了重组表达质粒ｐＥＴ－６Ｒ（Ｂ）和ＰＥＴ－６Ｒ（Ｂ）４，分别编码２８ｋＤ和ｈＩＬ－６Ｒ配基结合区片段及其５３ｋＤ的二联体蛋白，并为酶切分析和ＤＮＡ序列分析所证实，ＳＤＳ－ＰＡＧＥ分析......
试验选用6周龄左右的健康小鼠,取回盲肠部黏液进行大肠杆菌和乳酸杆菌的分离培养,在培养基中添加不同浓度（0、0.1、1、10、100mmol/......
构建了一组ＨＮＰ－１原核表达载体，其中有几个含ＳＤ或Ω序列的载体，在表达产物中检测到了ＨＮＰ－１的存在，说明宿主菌是否在任何情况下都降解外源小分子多肽......
Cloning,expression and purification of the ligand-binding region of human IL-6R in E.coli and its pr
The ligand-binding region of human IL-6R is taken as the target gene fragment to be clonedand expressed.With pET-3b as e......
目的：获得具有较多保守抗原表位的ＨＩＶ－１膜重组抗原，提高ＨＩＶ筛选ＥＬＩＳＡ试剂的质量。方法：利用分子克隆与ＰＣＲ方法，获得了新的ＨＩＶ－１膜重组抗原的基因克隆，编码ｇｐ１２０Ｃ端亲......
目的构建分泌表达α-环糊精葡萄糖基转移酶(α-Cyclodextrin Glycosyltransferase,α-CGTase)的重组菌株,并利用该酶液制备AA-2G。......
To amplify human era (Hera) gene, then express it in E.coli. Methods: Human era gene, after amplified by PCR and identif......
对2012-2015年分离于陕西榆林地区50株鸡源致病性E.coli进行8种常用喹诺酮类药物药物敏感性试验,为指导临床合理使用喹诺酮类药物......
Receptor-binding domain of SARS-Cov spike protein: Soluble expression in purification and functional
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Objective:To detect the various bacteriological agents and pathological changes in commercial layer chicken affected wit......
An Improved Strategy for Efficient Expression and Purification of Soluble HIV-1 Tat Protein in E.col
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2011年4月以来,德国多个地方肠出血性大肠杆菌((EHEC) 0104:H4感染和溶血性尿毒综合征(HUS)疫情暴发.本文概述本次疫情的概况以及......
灭菌在现代食品工业中占有重要的地位，传统的热杀菌方法具有不可避免的缺陷，因此受到了一定的限制。近年来，随着人们生活水平的提高和......
Wild-type MIC Distribution and Epidemiological Cut-off Value and Resistant Characteristics of Colist
The aim of the present study was to investigate minimum inhibitory concentration (MIC) distributions by broth microdilut......
抗生素因具有良好的抑菌、杀菌作用被广泛应用于医药、农业和畜牧业领域。然而,被使用的抗生素高达75%被人体或动物排出体外进入环......
目的急性呼吸窘迫综合症(Acute Respiratory Distress Syndrome,ARDS)和急性肺损伤(Acute Lung Injury,ALI)是一种常发生在重症病......
Expansin是近年来发现的迄今惟一能诱导热钝化的离体细胞壁伸展的细胞壁特异性植物内源蛋白质。在一些可侵染或消化植物组织的生命......
Efficient and Quick Inactivation of SARS Coronavirus and Other Microbes Exposed to the Surfaces of S
Objective To study the two metal catalysts Ag/Al2O3 and Cu/Al2O3 that interdict the transmission pathway for SARS and ot......
